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KMID : 0881720140290040340
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Journal of Food Hygiene and Safety
2014 Volume.29 No. 4 p.340 ~ p.346
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Detection and Differentiation of Intentional and Unintentional Mixture in Raw Meats Using Real-time PCR
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Kim Kyu-Heon
Kim Mi-Ra
Park Young-Eun
Kim Young-Sang
Lee Ho-Yeon
Park Yong-Choon
Kim Sang-Yub
Choi Jang-Duck
Jang Young-Mi
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Abstract
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In this study, the detection method was developed using real-time PCR to distinguish 4 species (bovine, porcine, horse, and chicken) of raw meats. The genes for distinction of species about meats targeted at 12S rRNA and 16S rRNA parts in mitochondrial DNA. Probes were designed to have a 5' FAM and a TAMRA at the 3' end. This study is to develop 4 species-specific primer and probes about raw materials and real-time PCR on 10 strains to observe the products of non-specific signal for similar species. As a result, any non-specific signal were not detected among each other. Real-time PCR method was developed for quantitation and identification of intentional and unintentional mixture in ground mixed meat (The difference of CT value between intentional mixture and 100% meat: ≤ 4 cycles, The difference of CT value between unintentional mixture and 100% meat: ≥ 6 cycles). The detection and differentiation of intentional and unintentional mixture in this study would be applied to food safety management for eradication of adulterated food distribution and protection of consumer's right.
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KEYWORD
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Real-time PCR (Polymerase Chain Reaction), Raw Meats, mt (mitochondrial) DNA
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